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Intermediate

Evidence for Evolution: Fossils and Extinction

4.6.3.4 Evidence for evolution·4.6.3.5 Fossils·4.6.3.6 Extinction

Aligned to the AQA 8461 specification

Level
Intermediate
Reading time
9 min
Published
16 June 2026
Updated
1 July 2026
On this page
  1. 1.Why the Theory of Evolution Is Accepted
  2. 2.Antibiotic Resistance as Evidence
  3. 3.What Fossils Are and How They Form
  4. 4.Worked Example: Identifying How a Fossil Formed
  5. 5.Why Early Fossils Are Scarce
  6. 6.Extinction and Its Causes
  7. 7.Common Exam Mistakes

Key takeaways

  • Evolution by natural selection is widely accepted because of a large body of evidence from genetics, the fossil record and observed changes such as antibiotic resistance.
  • Antibiotic resistance evolves because resistant bacteria already exist by chance mutation and the antibiotic only selects for them; it does not create resistance.
  • Fossils form in three ways: prevented decay, replacement of hard parts by minerals, and preserved traces such as footprints, burrows and rootlets.
  • Early fossils are scarce because many early organisms were soft-bodied and rarely fossilised, and geological activity destroyed most of the traces that did form.
  • Extinction is the permanent loss of all members of a species, and can be caused by new predators, new diseases, new competitors, environmental change or a single catastrophic event.

Why the Theory of Evolution Is Accepted

The theory of evolution by natural selection is now widely accepted by scientists. It became accepted because of the large amount of evidence that supports it, gathered from several independent sources.

When Charles Darwin first published his theory, it was not fully accepted. One reason was that the mechanism of inheritance was unknown — Darwin could not explain how characteristics were passed from parents to offspring. That gap has since been filled.

Three main lines of evidence now support evolution:

EvidenceWhat it shows
Inheritance through genesCharacteristics are passed to offspring in genes, providing the mechanism Darwin lacked
The fossil recordA record of how organisms have changed over millions of years
Antibiotic resistance in bacteriaEvolution by natural selection happening fast enough to observe directly

Key idea: A theory becomes accepted not from one proof, but from a large body of evidence pointing the same way. Evolution is supported by genetics, fossils and observed changes in living organisms.

This lesson covers the fossil and bacterial evidence, then extinction — the permanent loss of species over geological time.

Antibiotic Resistance as Evidence

Bacteria reproduce very quickly, so evolution by natural selection can be seen happening over a short time. This makes antibiotic resistance a powerful, observable piece of evidence for evolution.

Worked walk-through — how a resistant population evolves:

  1. Within a population of bacteria there is variation — random mutations mean a few bacteria happen to carry a gene that makes them resistant to an antibiotic.
  2. An antibiotic is used. It kills the non-resistant bacteria, but the resistant ones survive.
  3. The surviving resistant bacteria reproduce, passing the resistance gene to their offspring.
  4. Over generations, the proportion of resistant bacteria rises until the whole population is resistant.

This is natural selection: variation, a selection pressure (the antibiotic), differential survival, and inheritance of the advantageous gene.

Exam tip: The antibiotic does not create resistance. The mutation already exists by chance; the antibiotic only selects for the bacteria that already carry it. Saying "the antibiotic made them resistant" is a common error and is unlikely to score.

MRSA (methicillin-resistant Staphylococcus aureus) is a real example of bacteria that evolved resistance in this way.

What Fossils Are and How They Form

Fossils are the remains of organisms from millions of years ago, found in rocks. They give us direct evidence of what lived in the past and how organisms have changed over time.

There are three main ways fossils form:

Formation routeWhat happensTypical example
Prevented decayParts of the organism do not decay because one or more conditions needed for decay are absent (e.g. no oxygen, no moisture, low temperature, no suitable microorganisms)Mammoths preserved in ice; insects in amber
Mineral replacementAs the organism's hard parts slowly decay, they are replaced by minerals, forming a rock-like copy of the originalPetrified bones, shells and wood
Preserved tracesThe organism itself is not preserved, but traces it left behind areFootprints, burrows, rootlet traces

Definition: Decay happens when microorganisms break down dead material. It needs oxygen, moisture, warmth and microorganisms. Remove one of these and decay slows or stops — which is exactly why some remains survive long enough to fossilise.

Note that traces such as footprints are still classed as fossils even though no part of the actual organism remains.

Worked Example: Identifying How a Fossil Formed

Use the three routes to reason about a given specimen. The key is to ask what was preserved and how.

Scenario A — A dinosaur leg bone, now solid stone, found in sandstone. The original bone has been turned to a rock-like material. The hard part has been replaced by minerals as it decayed. This is the mineral replacement route.

Scenario B — A mosquito with intact soft body parts, sealed inside amber (hardened tree resin). The whole insect, including soft tissue, has survived. The amber sealed it away from oxygen and microorganisms, so decay could not take place. The organism's parts did not decay because a condition for decay was absent. This is the prevented decay route.

Scenario C — A line of three-toed prints pressed into mudstone, with no bones nearby. No part of the animal is present — only the marks it made while walking. These are preserved traces (footprints). This is the preserved trace route.

Exam tip: Read the clue in the question. "Replaced by minerals / turned to stone" → mineral replacement. "No oxygen / amber / ice / peat bog" → prevented decay. "Footprints / burrows / rootlets" → preserved traces.

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Why Early Fossils Are Scarce

The fossil record tells us much about how life developed, but it has a large gap at the very beginning. Scientists cannot be certain how life on Earth began, because there are very few valid fossils from the earliest periods.

There are two main reasons for this gap:

  • Many early organisms were soft-bodied. Soft tissue decays quickly and rarely fossilises, because there are no hard parts to be replaced by minerals or to leave clear traces. These organisms left few traces behind.
  • The traces that did form were largely destroyed. Over the enormous timescales involved, geological activity — such as movement of rock, heat, pressure and erosion — destroyed most of the early traces that existed.

Key idea: An incomplete fossil record is not evidence against evolution. It reflects how fossils form: soft-bodied organisms rarely fossilise, and ancient traces are often destroyed. Absence of early fossils is expected, not surprising.

This is also why the fossil record is described as incomplete: we have a sample of past life, not a full catalogue. We can still learn from fossils how much, or how little, different groups of organisms have changed as life developed on Earth.

Extinction and Its Causes

Extinction is the permanent loss of all members of a species — it occurs when there are no remaining individuals of that species still alive anywhere. Extinction is permanent: once the last individual dies, the species cannot return.

Several factors may contribute to a species becoming extinct. Often more than one acts at the same time.

FactorWhy it can cause extinctionExample
New predatorsA new predator hunts a species that has no defences against itGround-nesting birds wiped out by introduced rats or cats
New diseasesA new pathogen spreads through a species with no resistanceAmphibian populations lost to the chytrid fungus
New, more successful competitorsAnother species competes better for the same food, space or other resourcesA native species out-competed by an introduced one
Environmental change over geological timeSlow changes in climate, sea level or habitat over very long timescalesCooling climates reducing suitable habitat
A single catastrophic eventOne sudden event destroys habitats or food supplies on a huge scaleA massive volcanic eruption or an asteroid collision

Worked walk-through — match the factor to the scenario:

A small island has one species of flightless bird. Sailors arrive and release rats, which eat the birds' eggs. Within decades the bird is gone. The trigger is an organism that was not there before and now eats the species. This is a new predator. (You could also argue the rats are competitors for ground nests, but predation on eggs is the clearer driver here — justify whichever you choose using the detail in the question.)

Common Exam Mistakes

1. Saying the antibiotic "creates" resistance

The resistance mutation arises by chance before the antibiotic is used. The antibiotic only selects for bacteria that are already resistant. Describe it as selection, not creation.

2. Forgetting that traces count as fossils

Footprints, burrows and rootlet traces are fossils even though no part of the organism's body is preserved. Do not limit fossils to bones and shells.

3. Mixing up the three formation routes

Match the clue to the route: replaced by minerals (mineral replacement), conditions for decay absent such as no oxygen, ice or amber (prevented decay), and footprints/burrows/rootlets (preserved traces).

4. Treating the patchy fossil record as evidence against evolution

The gap exists because early organisms were soft-bodied and left few traces, and geological activity destroyed most of those traces. This explains the gap; it does not undermine evolution.

5. Defining extinction loosely

Extinction is the permanent loss of all members of a species — not "when a species becomes rare" or "endangered". State that no individuals remain alive.

6. Giving only one cause of extinction

The specification expects a range of contributing factors: new predators, new diseases, new competitors, environmental change over geological time, and a single catastrophic event such as a volcanic eruption or asteroid collision. List several and link each to the scenario.

Key terms

Fossil
The remains, or a trace, of an organism from millions of years ago, found in rocks.
Antibiotic resistance
The evolution by natural selection of bacteria that survive an antibiotic because they carry a resistance gene, then reproduce and pass it on.
Decay
The breakdown of dead material by microorganisms, which needs oxygen, moisture, warmth and microorganisms.
Mineral replacement
A fossil-forming route in which an organism's hard parts are slowly replaced by minerals, forming a rock-like copy.
Prevented decay
A fossil-forming route in which parts of an organism do not decay because a condition needed for decay, such as oxygen or moisture, is absent.
Preserved traces
A fossil-forming route in which the organism itself is not preserved but marks it left, such as footprints, burrows or rootlets, are.
Extinction
The permanent loss of all members of a species, when no individuals remain alive anywhere.
Natural selection
The process where variation, a selection pressure, differential survival and inheritance of an advantageous gene lead a population to change over generations.

Frequently asked questions

No. The resistance mutation arises by chance before the antibiotic is used. The antibiotic kills the non-resistant bacteria, but the resistant ones survive and reproduce, so it only selects for resistance that already exists. Saying the antibiotic made them resistant is a common error.

There are three main routes: parts of the organism do not decay when a condition for decay such as oxygen or moisture is absent (for example mammoths in ice or insects in amber), hard parts are replaced by minerals to form a rock-like copy, or traces such as footprints, burrows and rootlets are preserved.

Many early organisms were soft-bodied, so they decayed quickly and rarely fossilised because they had no hard parts. Over the enormous timescales involved, geological activity such as heat, pressure and erosion destroyed most of the traces that did form. This gap is expected, not evidence against evolution.

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